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bioRxiv · 10.1101/2020.02.13.948240

Optical Control of Cytokine Signaling via Bioinspired, Polymer-Induced Latency

Abstract

Cytokine signaling is challenging to study and therapeutically exploit as the effects of these protein are often pleiotropic. A subset of cytokines can, however, exert signal specificity via association with latency-inducing proteins which cage the cytokine until disrupted by discreet biological stimuli. Inspired by this precision, here we describe a strategy for synthetic induction of cytokine latency via modification with photo-labile polymers that mimic latency while attached, then restore protein activity in response to light, thus controlling the magnitude, duration, and location of cytokine signals. We characterize the high dynamic range of latent cytokine activity modulation and find that polymer-induced latency, alone, can prolong in vivo circulation and bias receptor subunit binding. We further show that protein de-repression can be achieved with near single-cell resolution and demonstrate the feasibility of transcutaneous photoactivation. Future extensions of this approach could enable multicolor, optical reprogramming of cytokine signaling networks and more precise immunotherapies.

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BibTeXRIS

Perdue, L. A., Do, P., David, C., Chyong, A., Kellner, A., Ruggieri, A., Kim, H. R., Salaita, K., Lesinski, G. B., Porter, C. C., Dreaden, E. C.. 2020-02-14. Optical Control of Cytokine Signaling via Bioinspired, Polymer-Induced Latency. https://doi.org/10.1101/2020.02.13.948240

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