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bioRxiv · 10.1101/2020.02.12.945790

Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis

Riching, A. S.·Danis, E.·Zhao, Y.·Cao, Y.·Chi, C.·Bagchi, R.·Klein, B.·Xu, H.·Kutateladze, T.·McKinsey, T.·Buttrick, P.·Song, K.
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Abstract

Direct reprogramming of fibroblasts into cardiomyocytes (CMs) represents a promising strategy to regenerate CMs lost after ischemic heart injury. Overexpression of GATA4, HAND2, MEF2C, TBX5, miR-1, and miR-133 (GHMT2m) along with transforming growth factor beta (TGF-{beta}) inhibition efficiently promotes reprogramming. However, the mechanisms by which TGF-{beta} blockade promotes cardiac reprogramming remain unknown. Here, we identify interactions between the histone H3 lysine 27 trimethylation (H3K27me3) - demethylase JMJD3, the SWI/SNF remodeling complex subunit BRG1, and cardiac transcription factors. Furthermore, canonical TGF-{beta} signaling regulates the interaction between GATA4 and JMJD3. TGF-{beta} activation impairs the ability of GATA4 to bind target genes and prevents demethylation of H3K27 at cardiac gene promoters during cardiac reprogramming. Finally, a mutation in GATA4 (V267M) exhibits reduced binding to JMJD3 and impaired cardiomyogenesis. Thus, we have identified an epigenetic mechanism wherein canonical TGF-{beta} pathway activation impairs cardiac gene programming by interfering with GATA4-JMJD3 interactions.

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Riching, A. S., Danis, E., Zhao, Y., Cao, Y., Chi, C., Bagchi, R., Klein, B., Xu, H., Kutateladze, T., McKinsey, T., Buttrick, P., Song, K.. 2020-02-12. Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis. https://doi.org/10.1101/2020.02.12.945790

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