Single-cell transcriptomic profile reveals macrophage heterogeneity in medulloblastoma and their treatment-dependent recruitment

Dang, M. T.; Gonzalez, M.; Gaonkar, K. S.; Rathi, K. S.; Young, P.; Arif, S.; Zhai, L.; Alam, M.; Devalaraja, S.; Tsun, T.; Folkert, I.; Raman, P.; Rokita, J. L.; Martinez, D.; Taroni, J.; Shapiro, J.; Greene, C. S.; Savonen, C.; Hakonarson, H.; Curran, T.; Haldar, M.

doi:10.1101/2020.02.12.945642

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bioRxiv · 10.1101/2020.02.12.945642

Single-cell transcriptomic profile reveals macrophage heterogeneity in medulloblastoma and their treatment-dependent recruitment

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Abstract

The role of macrophages in medulloblastoma, the most common malignant pediatric brain tumor, is unclear. Using single-cell RNA sequencing in a mouse model of sonic hedgehog medulloblastoma and analysis of bulk RNA sequencing of human medulloblastoma, we investigated macrophage heterogeneity. Our findings reveal differential recruitment of macrophages with molecular-targeted versus radiation therapy and identify an immunosuppressive monocyte-derived macrophages following radiation treatment of mouse medulloblastoma, uncovering potential strategies for immunomodulation as adjunctive therapy.

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Dang, M. T., Gonzalez, M., Gaonkar, K. S., Rathi, K. S., Young, P., Arif, S., Zhai, L., Alam, M., Devalaraja, S., Tsun, T., Folkert, I., Raman, P., Rokita, J. L., Martinez, D., Taroni, J., Shapiro, J., Greene, C. S., Savonen, C., Hakonarson, H., Curran, T., Haldar, M.. 2020-02-13. Single-cell transcriptomic profile reveals macrophage heterogeneity in medulloblastoma and their treatment-dependent recruitment. https://doi.org/10.1101/2020.02.12.945642

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