A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies

Borden, P. M.; Zhang, P.; Shivange, A. V.; Marvin, J. S.; Cichon, J.; Dan, C.; Podgorski, K.; Figueiredo, A.; Novak, O.; Tanimoto, M.; Shigetomi, E.; Lobas, M. A.; Kim, H.; Zhu, P. K.; Zhang, Y.; Zheng, W. S.; Fan, C.; Wang, G.; Xiang, B.; Gan, L.; Zhang, G.-X.; Guo, K.; Lin, L.; Cai, Y.; Yee, A.; Aggarwal, A.; Ford, C. P.; Rees, D. C.; Dietrich, D.; KHAKH, B. S.; Dittman, J. S.; Gan, W.-B.; Koyama, M. S.; Jayaraman, V.; Cheer, J. F.; Lester, H. A.; Zhu, J. J.; Looger, L. L.

doi:10.1101/2020.02.07.939504

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bioRxiv · 10.1101/2020.02.07.939504

A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies

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Abstract

Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all animals.

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Borden, P. M., Zhang, P., Shivange, A. V., Marvin, J. S., Cichon, J., Dan, C., Podgorski, K., Figueiredo, A., Novak, O., Tanimoto, M., Shigetomi, E., Lobas, M. A., Kim, H., Zhu, P. K., Zhang, Y., Zheng, W. S., Fan, C., Wang, G., Xiang, B., Gan, L., Zhang, G.-X., Guo, K., Lin, L., Cai, Y., Yee, A., Aggarwal, A., Ford, C. P., Rees, D. C., Dietrich, D., KHAKH, B. S., Dittman, J. S., Gan, W.-B., Koyama, M. S., Jayaraman, V., Cheer, J. F., Lester, H. A., Zhu, J. J., Looger, L. L.. 2020-02-08. A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies. https://doi.org/10.1101/2020.02.07.939504

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