A Combination of Two Human Monoclonal Antibodies Limits Fetal Damage by Zika Virus in Macaques

Van Rompay, K. K. A.; Coffey, L. L.; Kapoor, T.; Gazumyan, A.; Keesler, R. I.; Jurado, A.; Peace, A.; Agudelo, M.; Watanabe, J.; Usachenko, J.; Singapuri, A.; Immareddy, R.; Ardeshir, A.; Stuart, J. B.; Bournazos, S.; Ravetch, J. V.; Balderes, P. J.; Lorenz, I. C.; Esswein, S. R.; Keeffe, J.; Bjorkman, P. J.; Wang, Q.; Rice, C. M.; MacDonald, M. R.; Nussenzweig, M. C.; Robbiani, D. F.

doi:10.1101/2020.01.31.926899

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bioRxiv · 10.1101/2020.01.31.926899

A Combination of Two Human Monoclonal Antibodies Limits Fetal Damage by Zika Virus in Macaques

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Abstract

Human infection by Zika virus (ZIKV) during pregnancy can lead to vertical transmission and fetal aberrations, including microcephaly. Prophylactic administration of antibodies can diminish or prevent ZIKV infection in animal models, but whether passive immunization can protect nonhuman primates and their fetuses during pregnancy has not been determined. Z004 and Z021 are neutralizing monoclonal antibodies to domain III of the envelope (EDIII) of ZIKV. Together the two antibodies protect nonpregnant macaques against infection even after Fc modifications to prevent antibody-dependent enhancement in vitro (ADE) and extend their half-lives. Here we report on prophylactic co-administration of the Fc-modified antibodies to pregnant rhesus macaques challenged 3 times with ZIKV during first and second trimester. The two antibodies did not entirely eliminate maternal viremia but limited vertical transmission protecting the fetus from neurologic damage. Thus, maternal passive immunization with two antibodies to EDIII can shield primate fetuses from the harmful effects of ZIKV. Significance statementZika virus (ZIKV) infection during pregnancy can cause fetal abnormalities. Vaccines against ZIKV are under development, but because of potential safety concerns due to disease enhancing antibodies, and the time required by active immunization to induce protective antibodies, there is a need to explore alternative strategies. Recombinant monoclonal antibodies can be modified to prevent enhancement of infection, and thus could be an efficacious and safe alternative to vaccines to confer rapid protection. We show that prophylactic administration of two engineered antibodies, Z004 and Z021, to pregnant macaques partially protects against fetal neurologic damage and limits vertical transmission of ZIKV.

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Van Rompay, K. K. A., Coffey, L. L., Kapoor, T., Gazumyan, A., Keesler, R. I., Jurado, A., Peace, A., Agudelo, M., Watanabe, J., Usachenko, J., Singapuri, A., Immareddy, R., Ardeshir, A., Stuart, J. B., Bournazos, S., Ravetch, J. V., Balderes, P. J., Lorenz, I. C., Esswein, S. R., Keeffe, J., Bjorkman, P. J., Wang, Q., Rice, C. M., MacDonald, M. R., Nussenzweig, M. C., Robbiani, D. F.. 2020-02-02. A Combination of Two Human Monoclonal Antibodies Limits Fetal Damage by Zika Virus in Macaques. https://doi.org/10.1101/2020.01.31.926899

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