Super-resolved live-cell imaging using Random Illumination Microscopy

Mangeat, T.; Labouesse, S.; Allain, M.; Poincloux, R.; Bouissou, A.; Cantaloube, S.; Courtais, E.; Vega, E.; Li, T.; Guenole, A.; Rouviere, C.; Allard, S.; Campo, N.; Suzanne, M.; Wang, X.; Michaux, G.; Pinot, M.; Le Borgne, R.; Tournier, S.; Idier, J.; Sentenac, A.

doi:10.1101/2020.01.27.905083

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bioRxiv · 10.1101/2020.01.27.905083

Super-resolved live-cell imaging using Random Illumination Microscopy

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Abstract

Super-resolution fluorescence microscopy has been instrumental to progress in biology. Yet, the photo-induced toxicity, the loss of resolution into scattering samples or the complexity of the experimental setups curtail its general use for functional cell imaging. Here, we describe a new technology for tissue imaging reaching a 114nm/8Hz resolution at 30 {micro}m depth. Random Illumination Microscopy (RIM) consists in shining the sample with uncontrolled speckles and extracting a high-fidelity super-resolved image from the variance of the data using a reconstruction scheme accounting for the spatial correlation of the illuminations. Super-resolution unaffected by optical aberrations, undetectable phototoxicity, fast image acquisition rate and ease of use, altogether, make RIM ideally suited for functional live cell imaging in situ. RIM ability to image molecular and cellular processes in three dimensions and at high resolution is demonstrated in a wide range of biological situations such as the motion of Myosin II minifilaments in Drosophila.

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Mangeat, T., Labouesse, S., Allain, M., Poincloux, R., Bouissou, A., Cantaloube, S., Courtais, E., Vega, E., Li, T., Guenole, A., Rouviere, C., Allard, S., Campo, N., Suzanne, M., Wang, X., Michaux, G., Pinot, M., Le Borgne, R., Tournier, S., Idier, J., Sentenac, A.. 2020-01-27. Super-resolved live-cell imaging using Random Illumination Microscopy. https://doi.org/10.1101/2020.01.27.905083

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