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bioRxiv · 10.1101/2020.01.16.909200

Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism

Abstract

Plasma membranes fulfil many physiological functions. In polarized cells, different membrane compartments take on specialized roles, each being allocated correct amounts of membrane. The Drosophila tracheal system, an established tubulogenesis model, contains branched terminal cells with subcellular tubes formed by apical plasma membrane invagination. We show that apical endocytosis and late endosome-mediated trafficking determine the membrane allocation to the apical and basal membrane domains. Basal plasma membrane growth stops if endocytosis is blocked, whereas the apical membrane grows excessively. Plasma membrane is initially delivered apically, and then continuously endocytosed, together with apical and basal cargo. We describe an organelle carrying markers of late endosomes and multivesicular bodies (MVBs) that is abolished by inhibiting endocytosis, and which we suggest acts as transit station for membrane destined to be redistributed both apically and basally. This is based on the observation that disrupting MVB formation prevents growth of both compartments.

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BibTeXRIS

Mathew, R., Rios-Barrera, L. D., Machado, P., Schwab, Y., Leptin, M.. 2020-01-16. Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism. https://doi.org/10.1101/2020.01.16.909200

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