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bioRxiv · 10.1101/2020.01.14.906230

Gapless assembly of maize chromosomes using long read technologies

Dawe, K.·Liu, J.·Seetharam, A. S.·Chougule, K.·Ou, S.·Swentowsky, K. W.·Gent, J. I.·Llaca, V.·Woodhouse, M.·Manchanda, N.·Presting, G. G.·Kudrna, D.·Alabady, M.·Hirsch, C.·Fengler, K.·Ware, D.·Michael, T.·Hufford, M.
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Abstract

Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We used long read technologies and an optical map based approach to produce a maize genome assembly composed of only 63 contigs. The B73-Ab10 genome includes gapless assemblies of chromosome 3 (236 Mb) and chromosome 9 (162 Mb), multiple highly repetitive centromeres and heterochromatic knobs, and 53 Mb of the Ab10 meiotic drive haplotype.

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Dawe, K., Liu, J., Seetharam, A. S., Chougule, K., Ou, S., Swentowsky, K. W., Gent, J. I., Llaca, V., Woodhouse, M., Manchanda, N., Presting, G. G., Kudrna, D., Alabady, M., Hirsch, C., Fengler, K., Ware, D., Michael, T., Hufford, M.. 2020-01-15. Gapless assembly of maize chromosomes using long read technologies. https://doi.org/10.1101/2020.01.14.906230

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