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bioRxiv · 10.1101/2020.01.10.895037

Population Analysis of Extracellular Vesicles in Microvolumes of Biofluids

Maia, J.·Batista, S.·Couto, N.·Gregorio, A. C.·Bodo, C.·Elzanowska, J.·Strano Moraes, M. C.·Costa-Silva, B.
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Abstract

Extracellular Vesicles (EVs), membrane vesicles released by all cells, are emerging mediators of cell-cell communication. By carrying biomolecules from tissues to biofluids, EVs have attracted attention as non-invasive sources of clinical biomarkers in liquid biopsies. Although frequently employed for content characterization of EVs, the study of bulk preparations lacks information on sub-populations and the intrinsic heterogeneity of vesicles. Importantly, these strategies also difficult the characterization of EVs from small quantities of samples. We here present a Flow Cytometry strategy that enables detailed population analysis of EVs, at the same time decreasing sample volume requirements and accelerating the overall processing time. We show its unique application for quality control of isolates of EVs by comparing the proportion of vesicular and non-vesicular particles in samples prepared by different protocols. In addition, we demonstrate its suitability for the study of populations of EVs from samples characterized by challenging small volumes. To illustrate that, we perform longitudinal non-lethal analysis of EVs in mouse plasma and in single-animal collections of murine vitreous humor. By allowing for the analysis of EVs from minimal amounts of sample, our Flow Cytometry strategy has an unexplored potential in the study of EVs in clinical samples with intrinsically limited volumes. When compared to conventional methods, it also multiplies by several times the number of different analytes that can be studied from a single collection of biofluid.

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Maia, J., Batista, S., Couto, N., Gregorio, A. C., Bodo, C., Elzanowska, J., Strano Moraes, M. C., Costa-Silva, B.. 2020-01-10. Population Analysis of Extracellular Vesicles in Microvolumes of Biofluids. https://doi.org/10.1101/2020.01.10.895037

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