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bioRxiv · 10.1101/2020.01.08.899401

Mass Spectrometry Imaging of Cocaine, Drug Removal and Methylphenidate AlterPhospholipids in Drosophila Brain

Philipsen, M. H.·Phan, N. T.·Fletcher, J. S.·Ewing, A. G.
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Abstract

Cocaine dependence displays a broad impairment in cognitive performance including attention, learning and memory. To obtain a better understanding of the action of cocaine in the nervous system, and the relation between phospholipids and memory, we have investigated whether phospholipids recover in the brain following cocaine removal using the fly model, Drosophila. In addition, the effects of methylphenidate, a substitute medication for cocaine dependence, on fly brain lipids after cocaine abuse are also determined to see if it can rescue the lipid changes caused by cocaine. Time of flight secondary ion mass spectrometry with a (CO2)6000+ gas cluster ion beam was used to detect intact phospholipids. We show that cocaine has persistent effects, both increasing and decreasing the levels of specific phosphatidylethanolamines and phosphatidylinositols. These changes remain after cocaine withdrawal and are not rescued by methylphenidate. Cocaine is again shown to generally increase the levels of phosphatidylcholines in the fly brain; however, after drug withdrawal the abundance of these lipids returns to the original level and methylphenidate treatment of the flies following cocaine exposure enhances the reversal of the lipid level reducing them below the original control. The study provides insight into the molecular effects of cocaine and methylphenidate on brain lipids. We suggest that phosphatidylcholine could be a potential target for the treatment of cocaine abuse, as well as be a significant hallmark of cognition and memory loss with cocaine.

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Philipsen, M. H., Phan, N. T., Fletcher, J. S., Ewing, A. G.. 2020-01-09. Mass Spectrometry Imaging of Cocaine, Drug Removal and Methylphenidate AlterPhospholipids in Drosophila Brain. https://doi.org/10.1101/2020.01.08.899401

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