Tiled Reconstruction Improves Structured Illumination Microscopy

Hoffman, D. P.; Betzig, E.

doi:10.1101/2020.01.06.895318

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bioRxiv · 10.1101/2020.01.06.895318

Tiled Reconstruction Improves Structured Illumination Microscopy

Hoffman, D. P.·Betzig, E.

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Abstract

Structured illumination microscopy (SIM) is widely used for fast, long-term, live-cell super-resolution imaging. However, SIM images can contain substantial artifacts if the sample does not conform to the underlying assumptions of the reconstruction algorithm. Here we describe a simple, easy to implement, process that can be combined with any reconstruction algorithm to alleviate many common SIM reconstruction artifacts and briefly discuss possible extensions.

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Hoffman, D. P., Betzig, E.. 2020-01-06. Tiled Reconstruction Improves Structured Illumination Microscopy. https://doi.org/10.1101/2020.01.06.895318

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