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bioRxiv · 10.1101/2019.12.30.891499

Effect of high frequency and low intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway

Chen, W.-T.·Lin, G.-B.·Kuo, Y.-Y.·Hsieh, C.-H.·Lu, C.-H.·Sun, Y.-K.·Chao, C.-Y.
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Abstract

As the most common type of neurodegenerative diseases (NDDs), Alzheimers disease (AD) is thought to be caused mainly by the excessive aggregation of {beta}-amyloid protein (A{beta}). However, a growing number of studies have found that reactive oxygen species (ROS) play a key role in the onset and progression of AD. The present study aimed to probe the neuroprotective effect of high-frequency low-intensity pulsed electric field (H-LIPEF) for SH-SY5Y cells against hydrogen peroxide (H2O2) and A{beta}-induced cytotoxicity. By looking in a systematic way into the frequency- and amplitude-dependent neuroprotective effect of pulsed electric field (PEF), the study finds that H-LIPEF at 200 Hz produces the optimal protective effect for SH-SY5Y cells. The underlying mechanisms were confirmed to be due to the activation of extracellular signal-regulated kinase (ERK) pathway and the downstream prosurvival and antioxidant proteins. Because the electric field can be modified to focus on specific area in a non-contact manner, the study suggests that H-LIPEF holds great potential for treating NDDs, whose effect can be further augmented with the administering of drugs or natural compounds at the same time.

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Chen, W.-T., Lin, G.-B., Kuo, Y.-Y., Hsieh, C.-H., Lu, C.-H., Sun, Y.-K., Chao, C.-Y.. 2019-12-31. Effect of high frequency and low intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway. https://doi.org/10.1101/2019.12.30.891499

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