Optimization and functionalization of red-shifted rhodamine dyes

Grimm, J. B.; Tkachuk, A. N.; Choi, H.; Mohar, B.; Falco, N.; Patel, R.; Lippincott-Schwartz, J.; Brown, T. A.; Lavis, L. D.

doi:10.1101/2019.12.20.881227

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bioRxiv · 10.1101/2019.12.20.881227

Optimization and functionalization of red-shifted rhodamine dyes

Grimm, J. B.·Tkachuk, A. N.·Choi, H.·Mohar, B.·Falco, N.·Patel, R.·Lippincott-Schwartz, J.·Brown, T. A.·Lavis, L. D.

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Abstract

Expanding the palette of fluorescent dyes is vital for pushing the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups.

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Grimm, J. B., Tkachuk, A. N., Choi, H., Mohar, B., Falco, N., Patel, R., Lippincott-Schwartz, J., Brown, T. A., Lavis, L. D.. 2019-12-20. Optimization and functionalization of red-shifted rhodamine dyes. https://doi.org/10.1101/2019.12.20.881227

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