Differential Correlation of Serum BDNF and microRNA Content in Rats with Rapid or Late Onset of Heavy Alcohol Use

Ehinger, Y.; phamluong, K.; Darevesky, D.; Welman, M.; Moffat, J.; Sakhai, S.; Whiteley, E.; Berger, A.; Laguesse, S.; Farokhnia, M.; Leggio, L.; Lordkipanidze, M.; Ron, D.

doi:10.1101/2019.12.18.881680

bioRxiv · 10.1101/2019.12.18.881680

Differential Correlation of Serum BDNF and microRNA Content in Rats with Rapid or Late Onset of Heavy Alcohol Use

Ehinger, Y.·phamluong, K.·Darevesky, D.·Welman, M.·Moffat, J.·Sakhai, S.·Whiteley, E.·Berger, A.·Laguesse, S.·Farokhnia, M.·Leggio, L.·Lordkipanidze, M.·Ron, D.

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Abstract

Heavy alcohol use reduces the levels of the brain-derived neurotrophic factor (BDNF) in the prefrontal cortex of rodents through the upregulation of microRNAs targeting BDNF mRNA. In humans, an inverse correlation exists between circulating blood levels of BDNF and the severity of psychiatric disorders including alcohol abuse. Here, we set out to determine whether a history of heavy alcohol use produces comparable alterations in the blood of rats. We used an intermittent access to 20% alcohol using the 2-bottle choice paradigm (IA20%2BC), and measured circulating levels of BDNF protein and microRNAs in the serum of Long-Evans rats before and after 8-weeks of excessive alcohol intake. We observed that the drinking profile of heavy alcohol users is not unified; Whereas 70% of the rats gradually escalate their alcohol intake (Late Onset), 30% of alcohol users exhibit a very Rapid Onset of drinking (Rapid Onset). We found that serum BDNF levels are negatively correlated with alcohol intake in both Rapid Onset and Late Onset rats. In contrast, increased expression of the microRNAs (miRs) targeting BDNF, miR30a-5p, miR-195-5p, miR191-5p and miR206-3p, was detected only in the Rapid Onset rats. Finally, we report that the alcohol-dependent molecular changes are not due to alterations in platelet number. Our data suggest that rats exhibit both Late and Rapid Onset of alcohol intake. We further show that heavy alcohol use produces comparable changes in BDNF protein levels in both groups. However, circulating microRNAs are responsive to alcohol only in the Rapid Onset rats.

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Ehinger, Y., phamluong, K., Darevesky, D., Welman, M., Moffat, J., Sakhai, S., Whiteley, E., Berger, A., Laguesse, S., Farokhnia, M., Leggio, L., Lordkipanidze, M., Ron, D.. 2019-12-19. Differential Correlation of Serum BDNF and microRNA Content in Rats with Rapid or Late Onset of Heavy Alcohol Use. https://doi.org/10.1101/2019.12.18.881680

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