FK506-binding protein 13 expression is upregulated in interstitial lung disease and correlated with clinical severity: a potentially protective role.

Tat, V.; Ayaub, E. A.; Ayoub, A.; Vierhout, M.; Naiel, S.; Padwal, M.; Abed, S.; Mekhael, O.; Tandon, K.; Revill, S. D.; Yousof, T.; Bellaye, P.-S.; Kolb, P.; Dvorkin-Gheva, A.; Naqvi, A.; Cutz, J.-C.; Hambly, N.; Kato, J.; Vaughan, M.; Moss, J.; Kolb, M.; Ask, K.

doi:10.1101/2019.12.15.858340

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bioRxiv · 10.1101/2019.12.15.858340

FK506-binding protein 13 expression is upregulated in interstitial lung disease and correlated with clinical severity: a potentially protective role.

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Abstract

Pulmonary fibrosis is a progressive lung disease characterized by myofibroblast accumulation and excessive extracellular matrix deposition. Endoplasmic reticulum (ER) stress initiates the unfolded protein response (UPR), a cellular stress response pathway that has been implicated in both inflammatory and fibrotic processes. Here, we sought to investigate the role of the 13 kDa FK506-binding protein (FKBP13), an ER stress-inducible molecular chaperone, in various forms of pulmonary fibrosis. We first characterized the gene and protein expression of FKBP13 in lung biopsy samples from 24 patients with idiopathic pulmonary fibrosis (IPF) and 17 control subjects. FKBP13 expression was found to be elevated in the fibrotic regions of IPF lung tissues, and within this cohort, was correlated with declining forced vital capacity and dyspnea severity. FKBP13 expression was also increased in lung biopsies of patients with hypersensitivity pneumonitis, rheumatoid arthritis, and sarcoidosis-associated interstitial lung disease. We next evaluated the role of this protein using FKBP13-/- mice in a bleomycin model of pulmonary fibrosis. Animals were assessed for lung function and histopathology at different stages of lung injury including the inflammatory (Day 7), fibrotic (Day 21) and resolution (Day 50) phase. FKBP13-/- mice showed increased infiltration of inflammatory cells and cytokines at Day 7, increased lung elastance and fibrosis at Day 21, and impaired resolution of fibrosis at Day 50. These changes were associated with an increased number of cells that stained positive for TUNEL and cleaved caspase 3 in the FKBP13-/- lungs, indicating a heightened cellular sensitivity to bleomycin. Our findings suggest that FKBP13 is a potential biomarker for severity or progression of interstitial lung diseases, and that it has a biologically relevant role in protecting mice against bleomycin-induced injury, inflammation and fibrosis.

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Tat, V., Ayaub, E. A., Ayoub, A., Vierhout, M., Naiel, S., Padwal, M., Abed, S., Mekhael, O., Tandon, K., Revill, S. D., Yousof, T., Bellaye, P.-S., Kolb, P., Dvorkin-Gheva, A., Naqvi, A., Cutz, J.-C., Hambly, N., Kato, J., Vaughan, M., Moss, J., Kolb, M., Ask, K.. 2019-12-15. FK506-binding protein 13 expression is upregulated in interstitial lung disease and correlated with clinical severity: a potentially protective role.. https://doi.org/10.1101/2019.12.15.858340

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