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bioRxiv · 10.1101/200923

Tree-ensemble analysis tests for presence of multifurcations in single cell data

Macnair, W.·Roditi, L. D. V.·Ganscha, S.·Claassen, M.
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Abstract

We introduce TreeTop, an algorithm for single-cell data analysis to identify and assess statistical significance of branch points in biological processes with possibly multi-level branching hierarchies. We demonstrate branch point identification for processes with varying topologies, including T cell maturation, B cell differentiation and hematopoiesis. Our analyses are consistent with recent experimental studies suggesting a shallow hierarchy of differentiation events in hematopoiesis, rather than the classical multi-level hierarchy.

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Macnair, W., Roditi, L. D. V., Ganscha, S., Claassen, M.. 2017-10-10. Tree-ensemble analysis tests for presence of multifurcations in single cell data. https://doi.org/10.1101/200923

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