The number of orphans in yeast and fly is drastically reduced by using combining searches in both proteomes and genomes

Basile, W.; Elofsson, A.

doi:10.1101/185983

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The number of orphans in yeast and fly is drastically reduced by using combining searches in both proteomes and genomes

Basile, W.·Elofsson, A.

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Abstract

The identification of de novo created genes is important as it provides a glimpse on the evolutionary processes of gene creation. Potential de novo created genes are identified by selecting genes that have no homologs outside a particular species, but for an accurate detection this identification needs to be correct.\n\nGenes without any homologs are often referred to as orphans; in addition to de novo created ones, fast evolving genes or genes lost in all related genomes might also be classified as orphans. The identification of orphans is dependent on: (i) a method to detect homologs and (ii) a database including genes from related genomes.\n\nHere, we set out to investigate how the detection of orphans is influenced by these two factors. Using Saccharomyces cerevisiae we identify that best strategy is to use a combination of searching annotated proteins and a six-frame translation of all ORFs from closely related genomes. Using this strategy we obtain a set of 54 orphans in Drosophila melanogaster and 38 in Drosophila pseudoobscura, significantly less than what is reported in some earlier studies.

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Basile, W., Elofsson, A.. 2017-09-07. The number of orphans in yeast and fly is drastically reduced by using combining searches in both proteomes and genomes. https://doi.org/10.1101/185983

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