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bioRxiv · 10.1101/185504

BraCeR: Reconstruction of B-cell receptor sequences and clonality inference from single-cell RNA-sequencing

Lindeman, I.·Emerton, G.·Sollid, L. M.·Teichmann, S.·Stubbington, M. J. T.
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Abstract

Reconstruction of antigen receptor sequences from single-cell RNA-sequencing (scRNA-seq) data allows the linking of antigen receptor usage to the full transcriptomic identity of individual B lymphocytes, without having to perform additional targeted repertoire sequencing (Rep-seq). Here we report BraCeR (freely available at https://github.com/teichlab/bracer/), an extension of TraCeR [1], for reconstruction of paired full-length B-cell receptor sequences and inference of clonality from scRNA-seq data (Supplementary Note 1).

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Lindeman, I., Emerton, G., Sollid, L. M., Teichmann, S., Stubbington, M. J. T.. 2017-09-07. BraCeR: Reconstruction of B-cell receptor sequences and clonality inference from single-cell RNA-sequencing. https://doi.org/10.1101/185504

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