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bioRxiv · 10.1101/180943

Inhibition of p53 improves CRISPR/Cas-mediated precision genome editing

Haapaniemi, E.·Botla, S. K.·Persson, J.·Schmierer, B.·Taipale, J.
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Abstract

We report here that genome editing by CRISPR/Cas9 induces a p53-mediated DNA damage response and cell cycle arrest. Transient inhibition of p53 prevents this response, and increases the rate of homologous recombination more than five-fold. This provides a way to improve precision genome editing of normal cells, but warrants caution in using CRISPR for human therapies until the mechanism of the activation of p53 is elucidated.

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Haapaniemi, E., Botla, S. K., Persson, J., Schmierer, B., Taipale, J.. 2017-08-25. Inhibition of p53 improves CRISPR/Cas-mediated precision genome editing. https://doi.org/10.1101/180943

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