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bioRxiv · 10.1101/177949

The small non-coding vault RNA1-1 acts as a riboregulator of autophagy

Horos, R.·Alleaume, A.-M.·Kleinendorst, R.·Tarafder, A. K.·Schwarzl, T.·Zielonka, E. M.·Adak, A.·Castello, A.·Huber, W.·Sachse, C.·Hentze, M. W.
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Abstract

Vault RNAs (vtRNA) are small, 88-100nt non-coding RNAs found in many eukaryotes. Although they have been linked to drug resistance, apoptosis and nuclear transport, their function remains unclear. Here we show that a human vtRNA, RNA1-1, specifically binds to the autophagy receptor sequestosome-1/p62. Antisense-mediated depletion of vault RNA1-1 augments, whereas increased vault RNA1-1 expression restricts, autophagic flux in a p62-dependent manner. Bulk autophagy induced by starvation reduces the levels of vault RNA1-1 and the fraction of RNA-bound p62. These findings show that RNAs can act as riboregulators of biological processes by interacting with proteins, and assign a function to a vault RNA.

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Horos, R., Alleaume, A.-M., Kleinendorst, R., Tarafder, A. K., Schwarzl, T., Zielonka, E. M., Adak, A., Castello, A., Huber, W., Sachse, C., Hentze, M. W.. 2017-08-18. The small non-coding vault RNA1-1 acts as a riboregulator of autophagy. https://doi.org/10.1101/177949

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