Linear Assembly of a Human Y Centromere using Nanopore Long Reads

Jain, M.; Olsen, H. E.; Turner, D. J.; Stoddart, D.; Bulazel, K. V.; Paten, B.; Haussler, D.; Willard, H.; Akeson, M.; Miga, K. H.

doi:10.1101/170373

bioRxiv · 10.1101/170373

Linear Assembly of a Human Y Centromere using Nanopore Long Reads

Jain, M.·Olsen, H. E.·Turner, D. J.·Stoddart, D.·Bulazel, K. V.·Paten, B.·Haussler, D.·Willard, H.·Akeson, M.·Miga, K. H.

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Abstract

The human genome reference sequence remains incomplete due to the challenge of assembling long tracts of near-identical tandem repeats-in centromeric regions. To address this, we have implemented a nanopore sequencing strategy to generate high quality reads that span hundreds of kilobases of highly repetitive DNAs. Here, we use this advance to produce a sequence assembly and characterization of the centromeric region of a human Y chromosome.

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Jain, M., Olsen, H. E., Turner, D. J., Stoddart, D., Bulazel, K. V., Paten, B., Haussler, D., Willard, H., Akeson, M., Miga, K. H.. 2017-07-31. Linear Assembly of a Human Y Centromere using Nanopore Long Reads. https://doi.org/10.1101/170373

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