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bioRxiv · 10.1101/163212

Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix

Thompson, M.·Hrabak, E.
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Abstract

Whatman FTA (R)Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA (R)Cards and FTA (R)Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.\n\nMethod SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.

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Thompson, M., Hrabak, E.. 2017-07-13. Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix. https://doi.org/10.1101/163212

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