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bioRxiv · 10.1101/156075

Modulation of Ether-a-go-go related gene (ERG) current governs intrinsic persistent activity in rodent neocortical pyramidal cells

Cui, E. D.·Strowbridge, B.
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Abstract

While cholinergic receptor activation has long been known to dramatically enhance the excitability of cortical neurons, the cellular mechanisms responsible for this effect are not well understood. We used intracellular recordings in rat neocortical brain slices to assess the ionic mechanisms supporting persistent firing modes triggered by depolarizing stimuli following cholinergic receptor activation. We found multiple lines of evidence suggesting that a component of the underlying hyperexcitability associated with persistent firing reflects a reduction in the standing (leak) K+ current mediated by Ether-a-go-go-Related Gene (ERG) channels. Three chemically diverse ERG channel blockers (terfenadine, ErgToxin-1, and E-4031) abolished persistent firing and the underlying increase in input resistance in deep pyramidal cells in temporal and prefrontal association neocortex. Calcium accumulation during triggering stimuli appear to attenuate ERG currents, leading to membrane potential depolarization and increased input resistance, two critical elements generating persistent firing. Our results also suggest that ERG current normally governs cortical neuron responses to depolarizing stimuli by opposing prolonged discharges and by enhancing the post-stimulus repolarization. The broad expression of ERG channels and the ability of ERG blocks to abolish persistent firing evoked by both synaptic and intracellular step stimuli suggests modulation of ERG channels may underlie many forms of persistent activity observed in vivo.

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Cui, E. D., Strowbridge, B.. 2017-06-26. Modulation of Ether-a-go-go related gene (ERG) current governs intrinsic persistent activity in rodent neocortical pyramidal cells. https://doi.org/10.1101/156075

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