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bioRxiv · 10.1101/130575

Assessing species biomass contributions in microbial communities via metaproteomics

Kleiner, M.·Thorson, E.·Sharp, C. E.·Dong, X.·Liu, D.·Li, C.·Strous, M.
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Abstract

Assessment of microbial community composition is the cornerstone of microbial ecology. Microbial community composition can be analyzed by quantifying cell numbers or by quantifying biomass for individual populations. However, as cell volumes can differ by orders of magnitude, these two approaches yield vastly different results. Methods for quantifying cell numbers are already available (e.g. fluorescence in situ hybridization, 16S rRNA gene amplicon sequencing), yet methods for assessing community composition in terms of biomass are lacking.\n\nWe developed metaproteomics based methods for assessing microbial community composition using protein abundance as a measure for biomass contributions of individual populations. We optimized the accuracy and sensitivity of the method using artificially assembled microbial communities and found that it is less prone to some of the biases found in sequencing-based methods. We applied the method using communities from two different environments, microbial mats from two alkaline soda lakes and saliva from multiple individuals.

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Kleiner, M., Thorson, E., Sharp, C. E., Dong, X., Liu, D., Li, C., Strous, M.. 2017-04-25. Assessing species biomass contributions in microbial communities via metaproteomics. https://doi.org/10.1101/130575

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