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bioRxiv · 10.1101/119693

Can Human Embryonic Stem Cell-Derived Stromal Cells Serve A Starting Material For Myoblasts?

Ando, Y.·Saito, M.·Machida, M.·Noro, C.·Takahashi, M.·Toyoda, M.·Umezawa, A.
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Abstract

A large number of myocytes is necessary to treat intractable muscular disorders such as Duchenne muscular dystrophy with cell-based therapies. However, starting materials for cellular therapy products such as myoblasts, marrow stromal cells, menstrual blood-derived cells and placenta-derived cells have a limited lifespan and cease to proliferate in vitro. From the viewpoints of manufacturing and quality control, cells with a long lifespan are more suitable as a starting material. In this study, we generated stromal cells for future myoblast therapy from a working cell bank of human embryonic stem cells (ESCs). The ESC-derived CD105+ cells with extensive in vitro proliferation capability exhibited myogenesis and genetic stability in vitro. These results imply that ESC-derived CD105+ cells are another cell source for myoblasts in cell-based therapy for patients with genetic muscular disorders. Since ESCs are immortal, mesenchymal stromal cells generated from ESCs can be manufactured at a large scale in one lot for pharmaceutical purposes.

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Ando, Y., Saito, M., Machida, M., Noro, C., Takahashi, M., Toyoda, M., Umezawa, A.. 2017-03-23. Can Human Embryonic Stem Cell-Derived Stromal Cells Serve A Starting Material For Myoblasts?. https://doi.org/10.1101/119693

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