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bioRxiv · 10.1101/118919

BEARscc: Robustness Of Single-Cell Clusters Determined Using Simulated Technical Replicates

Severson, D. T.·Owen, R. P.·White, M. J.·Lu, X.·Schuster-Boeckler, B.
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Abstract

Technical variance is a major confounding factor in single-cell RNA sequencing, not least because it is not possible to replicate measurements on the same cell. We present BEARscc, a tool that uses RNA spike-in controls to simulate experiment-specific technical replicates. We demonstrate that the tool improves the unsupervised classification of cells and facilitates the biological interpretation of single-cell RNA-seq experiments.

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Severson, D. T., Owen, R. P., White, M. J., Lu, X., Schuster-Boeckler, B.. 2017-03-21. BEARscc: Robustness Of Single-Cell Clusters Determined Using Simulated Technical Replicates. https://doi.org/10.1101/118919

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