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bioRxiv · 10.1101/118505

Semisynthetic pH-Sensitive Fluorophores For Imaging Exocytosis And Endocytosis

Martineau, M.·Somasundaram, A.·Grimm, J. B.·Gruber, T. D.·Choquet, D.·Taraska, J. W.·Lavis, L. D.·Perrais, D.
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Abstract

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe semisynthetic pH-sensitive protein conjugates that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

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Martineau, M., Somasundaram, A., Grimm, J. B., Gruber, T. D., Choquet, D., Taraska, J. W., Lavis, L. D., Perrais, D.. 2017-03-25. Semisynthetic pH-Sensitive Fluorophores For Imaging Exocytosis And Endocytosis. https://doi.org/10.1101/118505

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