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bioRxiv · 10.1101/101923

DNA-dependent RNA cleavage by the Natronobacterium gregoryi Argonaute

Sunghyeok, Y.·Taegeun, B.·Kyoungmi, K.·Omer, H.·Seung Hwan, L.·Yoon Young, K.·Kang-In, L.·Seokjoong, K.·Jin-Soo, K.
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Abstract

We show here that, unlike most other prokaryotic Argonaute (Ago) proteins, which are DNA-guided endonucleases, the Natronobacterium gregoryi-derived Ago (NgAgo) can function as a DNA-guided endoribonuclease, cleaving RNA, rather than DNA, in a targeted manner. The NgAgo protein, in complex with 5-hydroxylated or 5-phosphrylated oligodeoxyribonucleotides (ODNs) of variable lengths, split RNA targets into two or more fragments in vitro, suggesting its physiological role in bacteria and demonstrating a potential for degrading RNA molecules such as mRNA or lncRNA in eukaryotic cells in a targeted manner.

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Sunghyeok, Y., Taegeun, B., Kyoungmi, K., Omer, H., Seung Hwan, L., Yoon Young, K., Kang-In, L., Seokjoong, K., Jin-Soo, K.. 2017-01-20. DNA-dependent RNA cleavage by the Natronobacterium gregoryi Argonaute. https://doi.org/10.1101/101923

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