Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2

Dvorak, P.; Bednar, D.; Vanacek, P.; Balek, L.; Eiselleova, L.; Stepankova, V.; Sebestova, E.; Kunova Bosakova, M.; Konecna, Z.; Mazurenko, S.; Kunka, A.; Horak, D.; Chaloupkova, R.; Brezovky, J.; Krejci, P.; Prokop, Z.; Damborsky, J.

doi:10.1101/100636

bioRxiv · 10.1101/100636

Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2

Dvorak, P.·Bednar, D.·Vanacek, P.·Balek, L.·Eiselleova, L.·Stepankova, V.·Sebestova, E.·Kunova Bosakova, M.·Konecna, Z.·Mazurenko, S.·Kunka, A.·Horak, D.·Chaloupkova, R.·Brezovky, J.·Krejci, P.·Prokop, Z.·Damborsky, J.

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Abstract

Fibroblast growth factors (FGFs) play numerous regulatory functions in complex organisms, and their corresponding therapeutic potential is of growing interest to academics and industrial researchers alike. However, applications of these proteins are limited due to their low stability in vivo and in vitro. Here we tackle this problem using a generalizable computer-assisted protein engineering strategy to create a unique modified FGF2 with nine mutations displaying unprecedented stability and uncompromised biological function.

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Dvorak, P., Bednar, D., Vanacek, P., Balek, L., Eiselleova, L., Stepankova, V., Sebestova, E., Kunova Bosakova, M., Konecna, Z., Mazurenko, S., Kunka, A., Horak, D., Chaloupkova, R., Brezovky, J., Krejci, P., Prokop, Z., Damborsky, J.. 2017-01-15. Computer-Assisted Engineering of Hyperstable Fibroblast Growth Factor 2. https://doi.org/10.1101/100636

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