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bioRxiv · 10.1101/096388

Quantitative mRNA Imaging Throughout the Entire Drosophila Brain

Long, X.·Colonell, J.·Wong, A. M.·Singer, R. H.·Lionnet, T.
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Abstract

We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid, multiplexable and does not require molecular amplification; it allows facile mRNA expression quantification with subcellular resolution on a standard confocal microscope. Using a custom Bessel Beam-Structured Illumination microscope (BB-SIM), we further demonstrate single-mRNA detection across the entire brain sample.

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Long, X., Colonell, J., Wong, A. M., Singer, R. H., Lionnet, T.. 2016-12-22. Quantitative mRNA Imaging Throughout the Entire Drosophila Brain. https://doi.org/10.1101/096388

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