Autophagy mitigates high-temperature injury during microsporogenesis in Arabidopsis thaliana

Abstract

Autophagy is one of the cellular processes that break down cellular components during senescence, starvation, and stress. The susceptibility of plant pollen development to high-temperature (HT) stress is well known, but the involvement of autophagy in HT injury is yet to be clarified. Here, we found that following transfer to 30 {degrees}C, all autophagy-deficient (atg) mutants (atg2-1, 5-1, 7-2, and 10-1) of Arabidopsis thaliana tested displayed visibly impaired pollen development and anther dehiscence. HT-induced male sterility significantly increased in the atg mutants, but the degree of HT-induced obstacles did not change between the wild type (WT) and mutants from the seedling stage to the bolting stage. Cytological analyses showed that 30 {degrees}C promoted autophagy and autolysosome formation in both anther wall cells and microspores in developing anthers of WT, but the atg5-1 mutant did not show completion of tapetum degeneration and microspore maturation. HT upregulated hydrogen peroxide and dehydroascorbate reductase 1 production in both WT and atg5-1 anthers, but the basal levels were already higher in the mutant. HT repressed expression of UNDEAD and its regulator MYB80, which are required for tapetal programmed cell death (PCD) for proper pollen development. Taken together, our results suggest that autophagy functions in tapetum degeneration and pollen development during HT-caused tapetal PCD abortion.\n\nHighlightsO_LIIn Arabidopsis, autophagy is not essential for completion of the life cycle under normal temperatures.\nC_LIO_LIHigh temperature (HT) stress induces autophagy in developing anther wall cells and microspores.\nC_LIO_LIAutophagy deficient atg mutants become almost completely male-sterile at moderate HT.\nC_LIO_LIAutophagy plays a role in tapetum degeneration and pollen development during HT-caused abortion of tapetal program cell death.\nC_LI