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bioRxiv · 10.1101/087072

Interpreting deep mutational scanning data resulting from selections on solid media

Klesmith, J. R.·Thorwall, S.·Whitehead, T. A.
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Abstract

Deep mutational scanning is now used in directed evolution experiments to quantify the change in frequency of a cellular variant in a mixed population. A key concern is the extent to which the enrichment of a variant in a population corresponds to a fitness metric like relative growth rate or survival percentage. We present here analytical equations converting the enrichment of a variant to fitness metrics for plate-based selections. Using isogenic and mixed cultures we show that growth rates and survival percentages correlate for antibiotic plate-based selections. These results are important for proper interpretation of data resulting from deep sequencing.

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Klesmith, J. R., Thorwall, S., Whitehead, T. A.. 2016-11-10. Interpreting deep mutational scanning data resulting from selections on solid media. https://doi.org/10.1101/087072

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