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bioRxiv · 10.1101/084350

Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria.

FALEYE, T. O. C.·Adewumi, M. O.·Adeoti, M. O.·David, O. M.·Oluyege, A. O.·Adeniji, J. A.·Famurewa, O.
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Abstract

BackgroundThe need to investigate the contribution of non-polio enteroviruses to acute flaccid paralysis (AFP) cannot be over emphasized as we move towards a poliovirus free world. Hence, we aim to identify non-polio enteroviruses recovered from the faeces of children diagnosed with AFP in Nigeria.\n\nMethodsNinety-six isolates, (95 unidentified and one previously confirmed Sabin poliovirus 3) recovered on RD cell culture from the stool of children <15 years old diagnosed with AFP in 2014 were analyzed. All isolates were subjected to RNA extraction, cDNA synthesis and three different PCR reactions (one panenterovirus 5'-UTR and two VP1 amplification assays). VP1 amplicons were then sequenced isolates identified.\n\nResults93.75% (90/96) of the isolates were detected by at least one of the three assays as an enterovirus. Precisely, 79.17% (76/96), 6.25% (6/96), 7.295% (7/96) and 6.25% (6/96) of the isolates were positive for both, positive and negative, negative and positive, as well as negative for both the 5'-UTR and VP1 assays, respectively. In this study, sixty-nine (69) of the 83 VP1 amplicons sequenced were identified as 27 different enterovirus types. The most commonly detected were CV-B3 (10 isolates) and EV-B75 (5 isolates). Specifically, one, twenty-four and two of the enterovirus types identified in this study belong to EV-A, EV-B and EV-C respectively.\n\nDiscussionThis study reports the circulating strains of 27 non-polio enterovirus types in Nigerian children with AFP in 2014 and Nigerian strains of CV-B2, CV-B4, E17, EV-B80, EV-B73, EV-B97, EV-B93, EV-C99 and EV-A120.

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FALEYE, T. O. C., Adewumi, M. O., Adeoti, M. O., David, O. M., Oluyege, A. O., Adeniji, J. A., Famurewa, O.. 2016-10-30. Non-polio enteroviruses in faeces of children diagnosed with acute flaccid paralysis in Nigeria.. https://doi.org/10.1101/084350

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