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bioRxiv · 10.1101/076919

Mapping protein function with CRISPR/Cas9-mediated mutagenesis

Katherine F Donovan·Mudra Hegde·Meagan Sullender·Emma W Vaimberg·Cory M Johannessen·David E Root·John G Doench
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Abstract

CRISPR/Cas9 screening has proven to be a versatile tool for genomics research. We describe a CRISPR/Cas9-mediated approach to mutagenesis, exploiting the allelic diversity generated by error-prone non-homologous end-joining (NHEJ) to identify gain-of-function alleles of the MAPK signaling pathway genes MEK1 and BRAF. These results illustrate a scalable technique to easily generate cell populations containing thousands of endogenous allelic variants of any gene or genes to map variant functions.

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Katherine F Donovan, Mudra Hegde, Meagan Sullender, Emma W Vaimberg, Cory M Johannessen, David E Root, John G Doench. 2016-09-23. Mapping protein function with CRISPR/Cas9-mediated mutagenesis. https://doi.org/10.1101/076919

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