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bioRxiv · 10.1101/073619

HiChIP: Efficient and sensitive analysis of protein-directed genome architecture

Maxwell R Mumbach·Adam J. Rubin·Ryan A. Flynn·Chao Dai·Paul A. Khavari·William J. Greenleaf·Howard Y. Chang
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Abstract

Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers input requirement over 100-fold relative to ChIA-PET. HiChIP of cohesin reveals multi-scale genome architecture with greater signal to background than in situ Hi-C. Thus, HiChIP adds to the toolbox of 3D genome structure and regulation for diverse biomedical applications.

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Maxwell R Mumbach, Adam J. Rubin, Ryan A. Flynn, Chao Dai, Paul A. Khavari, William J. Greenleaf, Howard Y. Chang. 2016-09-08. HiChIP: Efficient and sensitive analysis of protein-directed genome architecture. https://doi.org/10.1101/073619

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