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bioRxiv · 10.1101/073015

Natural Variation in Arabidopsis Cvi-0 Accession Uncovers Regulation of Guard Cell CO2 Signaling by MPK12

Liina Jakobson·Lauri Vaahtera·Kadri Tõldsepp·Maris Nuhkat·Cun Wang·Yuh-Shuh Wang·Hanna Hõrak·Ervin Valk·Priit Pechter·Yana Sindarovska·Jing Tang·Chuanlei Xiao·Yang Xu·Ulvi Gerst Talas·Maido Remm·Saijaliisa Kangasjärvi·M. Rob G. Roelfsema·Honghong Hu·Jaakko Kangasjärvi·Mart Loog·Julian I. Schroeder·Hannes Kollist·Mikael Brosché
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Abstract

Plant gas exchange is regulated by guard cells that form stomatal pores. Stomatal adjustments are crucial for plant survival; they regulate uptake of CO2 for photosynthesis, loss of water and entrance of air pollutants such as ozone. We mapped ozone hypersensitivity, more open stomata and stomatal CO2-insensitivity phenotypes of the Arabidopsis thaliana accession Cvi-0 to a single amino acid substitution in MAP kinase 12 (MPK12). In parallel we showed that stomatal CO2-insensitivity phenotypes of a mutant cis (CO2-insensitive) were caused by a deletion of MPK12. Lack of MPK12 impaired bicarbonate-induced activation of S-type anion channels. We demonstrated that MPK12 interacted with the protein kinase HT1, a central node in guard cell CO2 signaling, and that MPK12 can function as an inhibitor of HT1. These data provide a new function for plant MPKs as protein kinase inhibitors and suggest a mechanism through which guard cell CO2 signaling controls plant water management.

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Liina Jakobson, Lauri Vaahtera, Kadri Tõldsepp, Maris Nuhkat, Cun Wang, Yuh-Shuh Wang, Hanna Hõrak, Ervin Valk, Priit Pechter, Yana Sindarovska, Jing Tang, Chuanlei Xiao, Yang Xu, Ulvi Gerst Talas, Maido Remm, Saijaliisa Kangasjärvi, M. Rob G. Roelfsema, Honghong Hu, Jaakko Kangasjärvi, Mart Loog, Julian I. Schroeder, Hannes Kollist, Mikael Brosché. 2016-09-01. Natural Variation in Arabidopsis Cvi-0 Accession Uncovers Regulation of Guard Cell CO2 Signaling by MPK12. https://doi.org/10.1101/073015

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