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bioRxiv · 10.1101/065250

Design: An assay based on single-polypeptide-chain heterodimeric A2AR/D2R and non-oligomerized fusions for in vivo analysis of their allosteric receptor-receptor interactions

Toshio KAMIYA·Takashi Masuko·Dasiel Oscar Borroto-Escuela·Haruo Okado·Hiroyasu Nakata
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Abstract

BackgroundThe adenosine A2A receptor (A2AR) heteromerizes with the dopamine D2 receptor (D2R). In order to explore their functional interaction, we engineered previously stable single-polypeptide-chain (sc) A2AR/D2LR: whether the molecular entity of the striatal A2AR/D2R antagonism, i.e., scA2AR/D2Rs are just A2AR/D2R with the antagonism, remains unresolved.\n\nNew MethodTo further clarify the heteromerization through the scA2AR/D2LR, we here designed supramolecularly exclusive monomers and dimers, using the C{varepsilon}2 domain of IgE-Fc or apoproteins of the bacterial light-harvesting antenna complex.\n\nResultsA concept of the recptor protein assembly regulation, i.e., the selective monomer/non-obligate dimer formation was obtained. Although none of these new fusions were constructed or tested, we could aim at obtaining heterodimer-specific agents, using the scA2AR/D2R. Whether the resulting designs were explained feasibly and rationally was addressed. The structure and function of the non-obligate dimer were here discussed through scA2AR/D2R, focusing on the procedure of the membrane protein design and methods for transient protein-protein interactions.\n\nSummary and OutlookGiven that upon being expressed and allosteric regulation occurs regardless of specific signal to non-specific noise (S/N) ratio, the supramolecular designs, allowing us to express selectively monomer/non-obligate dimer of class A GPCR, are experimentally testable and will be used to confirm in vivo that such low S/N ratio interaction between A2AR and D2LR functions in the dopamine neurotransmission in the striatum.\n\nAbbreviations

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Toshio KAMIYA, Takashi Masuko, Dasiel Oscar Borroto-Escuela, Haruo Okado, Hiroyasu Nakata. 2016-07-26. Design: An assay based on single-polypeptide-chain heterodimeric A2AR/D2R and non-oligomerized fusions for in vivo analysis of their allosteric receptor-receptor interactions. https://doi.org/10.1101/065250

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