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bioRxiv · 10.1101/041384

Diffusion pseudotime robustly reconstructs lineage branching

Laleh Haghverdi·Maren Buettner·F. Alexander Wolf·Florian Buettner·Fabian J. Theis
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Abstract

Single-cell gene expression profiles of differentiating cells encode their intrinsic latent temporal order. We describe an efficient way to robustly estimate this order according to a diffusion pseudotime, which measures transitions on all length scales between cells using diffusion-like random walks. This allows us to identify cells that undergo branching decisions or are in metastable states, and thereby genes differentially regulated at these states.

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Laleh Haghverdi, Maren Buettner, F. Alexander Wolf, Florian Buettner, Fabian J. Theis. 2016-02-29. Diffusion pseudotime robustly reconstructs lineage branching. https://doi.org/10.1101/041384

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