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bioRxiv · 10.1101/037937

Validating silicon polytrodes with paired juxtacellular recordings: method and dataset

Joana P. Neto·Gonçalo Lopes·João Frazão·Joana Nogueira·Pedro Lacerda·Pedro Baião·Arno Aarts·Alexandru Andrei·Silke Musa·Elvira Fortunato·Pedro Barquinha·Adam Kampff
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Abstract

Cross-validating new methods for recording neural activity is necessary to accurately interpret and compare the signals they measure. Here we describe a procedure for precisely aligning two probes for in vivo \"paired-recordings\" such that the spiking activity of a single neuron is monitored with both a dense extracellular silicon polytrode and a juxtacellular micro-pipette. Our new method allows for efficient, reliable, and automated guidance of both probes to the same neural structure with micron resolution. We also describe a new dataset of paired-recordings, which is available online. We propose that our novel targeting system, and ever expanding cross-validation dataset, will be vital to the development of new algorithms for automatically detecting/sorting single-units, characterizing new electrode materials/designs, and resolving nagging questions regarding the origin and nature of extracellular neural signals.

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Joana P. Neto, Gonçalo Lopes, João Frazão, Joana Nogueira, Pedro Lacerda, Pedro Baião, Arno Aarts, Alexandru Andrei, Silke Musa, Elvira Fortunato, Pedro Barquinha, Adam Kampff. 2016-01-26. Validating silicon polytrodes with paired juxtacellular recordings: method and dataset. https://doi.org/10.1101/037937

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