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bioRxiv · 10.1101/035071

Nested metabarcode tagging: a robust tool for studying species interactions in ecology and evolution

James John Neil Kitson·Christoph Hahn·Richard J Sands·Nigel A Straw·Darren M Evans·David H Lunt
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Abstract

Determining the host-parasitoid interactions and parasitism rates for invasive species entering novel environments is an important first step in assessing potential routes for biocontrol and integrated pest management. Conventional insect rearing techniques followed by taxonomic identification are widely used to obtain such data, but this can be time consuming and prone to biases. Here we present a Next Generation Sequencing approach for use in ecological studies which allows for individual level metadata tracking of large numbers of invertebrate samples through the use of hierarchically organised molecular identification tags. We demonstrate its utility using a sample data set examining both species identity and levels of parasitism in late larval stages of the Oak Processionary Moth (Thaumetopoea processionea - Linn. 1758), an invasive species recently established in the UK. Overall we find that there are two main species exploiting the late larval stages of Oak Processionary Moth in the UK with the main parasitoid (Carcelia iliaca-Ratzeburg, 1840) parasitising 45.7% of caterpillars, while a rare secondary parasitoid (Compsilura conccinata-Meigen, 1824) was also detected in 0.4% of caterpillars. Using this approach on all life stages of the Oak Processionary Moth may demonstrate additional parasitoid diversity. We discuss the wider potential of nested tagging DNA-metabarcoding for constructing large, highly-resolved species interaction networks.

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James John Neil Kitson, Christoph Hahn, Richard J Sands, Nigel A Straw, Darren M Evans, David H Lunt. 2015-12-23. Nested metabarcode tagging: a robust tool for studying species interactions in ecology and evolution. https://doi.org/10.1101/035071

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