Protein degradation in a TX-TL cell-free expression system using ClpXP protease

Abstract

Introduction Introduction RESULTS AND DISCUSSION CONCLUSION ACKNOWLEDGEMENTS MATERIALS AND METHODS REFERENCES An in vitro S30-based Escherichia coli expression system (\"Transcription-Translation\", or \"TX-TL\") has been developed as an alternative prototyping environment to the cell for synthetic circuits [1-5]. Basic circuit elements, such as switches and cascades, have been shown to function in TX-TL, as well as bacteriophage assembly [2, 6]. Circuits can also be prototyped from basic parts within 8 hours, avoiding cloning and transformation steps [7]. However, most published results have been obtained in a \"batch mode\" reaction, where factors that play an important role for in vivo circuit dynamics ...