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bioRxiv · 10.1101/016451

Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud

Michael A. Cianfrocco·Andres E. Leschziner
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Abstract

The advent of a new generation of electron microscopes and direct electron detectors has realized the potential of single particle cryo-electron microscopy (cryo-EM) as a technique to generate high-resolution structures. However, calculating these structures requires high performance computing clusters, a resource that may be limiting to many likely cryo-EM users. To address this limitation and facilitate the spread of cryo-EM, we developed a publicly available off-the-shelf computing environment on Amazons elastic cloud computing infrastructure. This environment provides users with single particle cryo-EM software packages and the ability to create computing clusters that can range in size from 16 to 480+ CPUs. Importantly, these computing clusters are also cost-effective, as we illustrate here by determining a near-atomic resolution structure of the 80S yeast ribosome for $28.89 USD in ~10 hours.

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Michael A. Cianfrocco, Andres E. Leschziner. 2015-03-14. Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud. https://doi.org/10.1101/016451

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