Search bioRxiv⌕ Search

bioRxiv · 10.1101/012716

The utility of visual estimation in the rapid assessment of grass abundance for studies of foraging

Abstract

QuestionsTo assess the feasibility of using visually-estimated cover in rapid assessment of herbivore food species abundance in the grass layer, we asked the following questions: 1) What is the relationship between total graminoid cover and biomass in forests, and does height improve the prediction of biomass from cover? 2) How does total cover relate to biomass in a grassland habitat? 3) How does elephant food species graminoid cover relate to individual species biomass? 4) How well does species diversity of forest understorey grass layer, calculated from cover data, mirror that calculated from biomass data?\n\nLocationNagarahole National Park, India.\n\nMethodsWe estimated the abundance of graminoids through visual estimation of cover and weighted harvested biomass in forest and grassland plots. In forests, two estimates of total graminoid abundance, total graminoid cover and sum of species covers, were used. In the grassland, only total graminoid abundance was measured. We examined the relationship between cover estimates and biomass, and the additional utility of height in predicting biomass, using multiple regressions and AIC-based model selection. We also assessed similarity in cover- and biomass-based Simpsons and Shannon-Wiener diversity indices using regressions.\n\nResultsGraminoid cover explained a large portion of variation in total graminoid biomass in forest and grassland habitats. The sum of species covers was better than total cover in estimating total graminoid biomass in the forest. The benefit of including height to estimate total biomass was moderate in forests but substantial in grassland. Cover estimates were good proxies of food species biomass, and the addition of height did not yield better models for most species. Species diversity indices calculated from cover largely matched those based on biomass.\n\nConclusionsVisual estimation of species cover is a good alternative to biomass harvesting for rapid assessment of abundance of graminoids consumed by generalist herbivores, like elephants.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Hansraj Gautam, G.G. Potdar, T.N.C. Vidya. 2014-12-13. The utility of visual estimation in the rapid assessment of grass abundance for studies of foraging. https://doi.org/10.1101/012716

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

Species-dependent accumulation of PsaA in etioplasts points to light-independent steps in Photosystem I biogenesis

Whether Photosystem I (PSI) core subunits accumulate prior to light exposure in developing angiosperm seedlings remains unresolved, with conflicting reports across species. Here, we investigated the presence and membrane colocalization of the PSI core subunit PsaA in etioplasts of dark-grown angiosperms representing dicot and monocot species. Immunoblotting showed that PsaA accumulates in etioplasts of all three dicot species examined (pea, Arabidopsis, and runner bean), whereas in the monocot oat it was detected only after prolonged etiolation, at substantially lower levels and with an anomalously high apparent molecular weight. Blue-native PAGE analysis reveals that a fraction of PsaA co-migrates with LPOR, PsaB, FNR, and chlorophyll synthase, suggesting co-localization within a shared membrane microdomain rather than stable complex formation. The thylakoid insertase Alb3 was more abundant in dicot etioplasts, consistent with a potential role in the early integration of PsaA into the membrane. Upon illumination, pea reached PSI functionality faster than oat, with P700 oxidation detectable 30 min earlier, linking the dark accumulation of PsaA to an accelerated photosynthetic onset. These findings demonstrate light-independent accumulation of a PSI core subunit in a species-dependent manner and point to early steps in PSI biogenesis that precede full photosynthetic complex assembly. HighlightContrary to prevailing models, a Photosystem I core subunit PsaA accumulates in dark-grown angiosperm seedlings before light exposure, revealing light-independent early steps in photosynthetic complex biogenesis.

Preprint↗

Discover Novel RNA Targeting Small Molecules by Fluorescent Aptamer Screening

Discovering small molecules targeting proteins represents a major effort in drug development. RNA, however, as a class of macromolecule that carrying out important regulatory roles in the cell as drug target, only received attention recently. Although several methods have been proposed, an easy to operate, fast and robust method is still lacking. We designed a generic florescence screening method by fusing the target RNA with a florescent aptamer (fusion RNA) and then carried out screening using high-throughput format (Fluorescent Aptamer Screening, FAS). In this work, we chose SL5 on SARS-Cov-2 5UTR as the test target. SL5 is a conserved motif across several corona virus family members whose core is not prone to mutation. We screened 9528 compounds, successfully identified four molecules (Sertraline (hydrochloride), Samuraciclib (hydrochloride), Minocycline (hydrochloride), JG-98 bind direct to the full-length SL5 at micromolar or higher affinity. The design of FAS could be easily adapted to structured RNA motifs without prior knowledge of its 3D structural information. In addition, this work showed the possibility of developing generic drugs for RNA virus by targeting the conserved viral RNA genome and paved a new way for the discovery of small molecule drugs in combating human diseases.

Preprint↗

Mechanism and key RNA determinants of SARS-CoV-2 Nsp1-induced endonucleolytic cleavage of mRNA

SARS-CoV-2 nonstructural protein 1 (Nsp1) binds to 40S ribosomal subunits and induces host protein synthesis shut off by inhibiting translation initiation and triggering endonucleolytic cleavage of cellular mRNAs. Irrespective of the mode of initiation, Nsp1-mediated cleavage is induced by the cooperative action of the N-terminal domain of Nsp1, the RRM domain of eIF3g and 40S subunits. Using in vitro reconstitution, we determined that cleavage occurs by transesterification following intramolecular nucleophilic attack of the 2OH of the ribose on the adjacent phosphodiester bond yielding 5OH and 2,3 -cyclic phosphate termini. Cleavage requires a guanosine [~]10-22 nucleotides from the 5 end of mRNA, occurs within a narrow window upstream of this G, is most efficient between nucleotides at positions -6/-7 and -7/-8 relative to G, and shows a preference for Pu at positions -7 or -8 which provides the 2OH for the nucleophilic attack. Zero-length UV cross-linking of Nsp1 to nucleotides at positions -1 and -2 suggests that the critical guanosine may be recognized by Nsp1. Resistance to Nsp1-mediated cleavage of SARS-CoV-2 mRNA was ensured both by the relatively long distance between its G23G24 and the 5end and by the preceding oligoPy stretch lacking purines at positions -7 or -8 upstream of G23G24.

Preprint↗