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bioRxiv · 10.1101/006726

Improved vectors and genome-wide libraries for CRISPR screening

Neville E Sanjana·Ophir Shalem·Feng Zhang
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Abstract

Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)-associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and have been used to reveal new mechanisms in diverse biological models1-4. Previously, we used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations conferring vemurafenib resistance in a melanoma model1. However, initial lentiviral delivery systems for CRISPR screening had low viral titer or required a cell line already expressing Cas9, limiting the range of biological systems amenable to screening.

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Neville E Sanjana, Ophir Shalem, Feng Zhang. 2014-06-28. Improved vectors and genome-wide libraries for CRISPR screening. https://doi.org/10.1101/006726

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