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bioRxiv · 10.1101/004499

In vivo generation of DNA sequence diversity for cellular barcoding

Ian Peikon·Diana Gizatullina·Anthony Zador
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Abstract

Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic \"barcode\" that can be recovered by DNA sequencing. We demonstrate the feasibility of this technique in bacterial cells. This method should prove useful in tracking interactions of cells within a network, and/or heterogeneity within complex biological samples.

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Ian Peikon, Diana Gizatullina, Anthony Zador. 2014-04-24. In vivo generation of DNA sequence diversity for cellular barcoding. https://doi.org/10.1101/004499

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