MicroRNAs Regulate the Wnt/Ca2+ Signaling Pathway to Promote the Secretion of Insulin in Pancreatic Nestin-Positive Progenitor Cells

Chunyu Bai; Xiangchen Li; Yuhua Gao; Taofeng Lu; Kunfu Wang; Qian Li; Hui Xiong; Jia Chen; Ping Zhang; Wenjie Wang; Tingting Sun; Zhiqiang Shan; Bo Sun; Pei Pei; Changqing Liu; Weijun Guan; Yuehui Ma

doi:10.1101/003913

bioRxiv · 10.1101/003913

MicroRNAs Regulate the Wnt/Ca2+ Signaling Pathway to Promote the Secretion of Insulin in Pancreatic Nestin-Positive Progenitor Cells

Chunyu Bai·Xiangchen Li·Yuhua Gao·Taofeng Lu·Kunfu Wang·Qian Li·Hui Xiong·Jia Chen·Ping Zhang·Wenjie Wang·Tingting Sun·Zhiqiang Shan·Bo Sun·Pei Pei·Changqing Liu·Weijun Guan·Yuehui Ma

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Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that bind to the 3'-UTR of mRNAs and function mainly in post-transcriptional regulation. MiRNAs have been implicated to play roles in organ development, including that of the pancreas. Many miRNAs, such as miR-375, miR-124, miR-7, miR-21 and miR-221, have been shown to regulate insulin production as well as insulin secretion. However, it is not known whether miRNAs can regulate insulin secretion via the control of intracellular Ca2+ in pancreatic beta cells. In this research, expression profiles of miRNAs and mRNAs were investigated using RNA-sequencing and microarray analysis in chicken pancreatic nestin-positive progenitor cells and differentiated pancreatic beta cells. A number of miRNAs were up-regulated after differentiation of progenitors into beta cells, which regulate cell signaling pathways to control cell function. miR-223 and miR146a were shown to promote insulin secretion from pancreatic beta cells by regulating the concentration of intracellular Ca2+ via the down-regulation of their target genes.

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Chunyu Bai, Xiangchen Li, Yuhua Gao, Taofeng Lu, Kunfu Wang, Qian Li, Hui Xiong, Jia Chen, Ping Zhang, Wenjie Wang, Tingting Sun, Zhiqiang Shan, Bo Sun, Pei Pei, Changqing Liu, Weijun Guan, Yuehui Ma. 2014-04-04. MicroRNAs Regulate the Wnt/Ca2+ Signaling Pathway to Promote the Secretion of Insulin in Pancreatic Nestin-Positive Progenitor Cells. https://doi.org/10.1101/003913

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