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bioRxiv · 10.1101/002006

Genome-wide patterns of copy number variation in the diversified chicken genomes using next-generation sequencing

Guoqiang Yi·Lujiang Qu·Jianfeng Liu·Yiyuan Yan·Guiyun Xu·Ning Yang
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Abstract

Copy number variation (CNV) is important and widespread in the genome, and is a major cause of disease and phenotypic diversity. Herein, we perform genome-wide CNV analysis in 12 diversified chicken genomes based on whole genome sequencing. A total of 9,025 CNV regions (CNVRs) covering 100.1 Mb and representing 9.6% of the chicken genome are identified, ranging in size from 1.1 to 268.8 kb with an average of 11.1 kb. Sequencing-based predictions are confirmed at high validation rate by two independent approaches, including array comparative genomic hybridization (aCGH) and quantitative PCR (qPCR). The Pearson?s correlation values between sequencing and aCGH results range from 0.395 to 0.740, and qPCR experiments reveal a positive validation rate of 91.71% and a false negative rate of 22.43%. In total, 2,188 predicted CNVRs (24.2%) span 2,182 RefSeq genes (36.8%) associated with specific biological functions. Besides two previously accepted copy number variable genes EDN3 and PRLR, we also find some promising genes with potential in phenotypic variants. FZD6 and LIMS1, two genes related to diseases susceptibility and resistance are covered by CNVRs. Highly duplicated SOCS2 may lead to higher bone mineral density. Entire or partial duplication of some genes like POPDC3 and LBFABP may have great economic importance in poultry breeding. Our results based on extensive genetic diversity provide the first individualized chicken CNV map and genome-wide gene copy number estimates and warrant future CNV association studies for important traits of chickens.

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Guoqiang Yi, Lujiang Qu, Jianfeng Liu, Yiyuan Yan, Guiyun Xu, Ning Yang. 2014-01-23. Genome-wide patterns of copy number variation in the diversified chicken genomes using next-generation sequencing. https://doi.org/10.1101/002006

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