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zhang, r.

Publications and source records attributed to zhang, r..

4 recordsLinked to original sources

AffectRoute: Role-Structured EEG and Peripheral Physiology for Subject-Independent Affect Regression

Subject-independent affect regression from physiological signals remains challenging because emotional responses vary substantially across individuals, widely used datasets provide only coarse trial-level annotations, and heterogeneous physiological modalities may not contribute reliably when treated as if they were interchangeable predictors. We have developed AffectRoute, a protocol-conditioned subject-independent affect regression that is conditioned on the protocol and assigns separate predictive functions to information from the population, electroencephalography (EEG), and peripheral physiological signals (PPS). First, a source-population prior establishes a trial-level affective anchor using only the data from source participants. TrajBridge then combines an EEG representation that is supervised by REFED for participant-specific adjustments with temporal structure obtained from the continuous REFED annotations in order to create a weakly supervised segment-resolved pseudo-trajectory and to establish a frozen trial-level baseline. PhysioRoute next reduces the remaining error by breaking down the residual correction into a source-derived direction, which is estimated from the out-of-fold residuals within the source group, and a channel-specific magnitude derived from the PPS. When evaluated on DEAP and DREAMER using a leave-one-subject-out approach at the participant level, AffectRoute showed consistent step-by-step improvements in both the mean absolute error and the concordance correlation coefficient. A method that relied solely on the source data was clearly worse than PhysioRoute, showing that the final improvement cannot be accounted for by transferable source residual regularity alone. Conventional alternatives to fusing the PPS were also found to be consistently less effective, although analyses at the channel level and with a leave-one-channel-out design showed that the peripheral contributions are axis-dependent yet distributed across channels. These results indicate that structured residual inference is an effective alternative to unrestricted multimodal fusion for subject-independent affect regression.

neuroscience↗

The effect of melittin intervention on murine cervical cancer cells: An in-depth proteomics investigation

Melittin, the principal bioactive peptide of bee venom, exhibits promising antitumor activity, whereas its molecular mechanisms in cervical cancer remain incompletely understood. In this study, the biological effects and molecular responses of melittin in U14 cervical cancer cells were investigated using Astral data-independent acquisition (Astral-DIA)-based quantitative proteomics combined with molecular validation. The effects of melittin on cell migration, invasion, and cell death were evaluated by Transwell assays and PI/Hoechst staining. Differentially expressed proteins (DEPs) were screened and subjected to Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) analyses. Representative oxidative stress-related genes and proteins were further validated by RT-qPCR and Western blotting. Melittin significantly inhibited the migration and invasion of U14 cervical cancer cells and increased cell death. Quantitative proteomics identified 9,782 protein groups and 187 DEPs, including 71 up- and 116 down-regulated proteins. KEGG pathway enrichment analysis revealed oxidative phosphorylation (OXPHOS) as the most significantly enriched pathway, together with glutathione metabolism, ferroptosis-related pathways, reactive oxygen species signaling, and mitophagy. GO term enrichment analysis indicated that DEGs were mainly engaged in mitochondrial function, electron transport, oxidoreductase activity, and energy metabolism. RT-qPCR assay demonstrated altered expression of Duox1, Gpx4, Gsx2, Nfe2l2, and Gstp2. Additionally, PPI analysis identified Gstp2 and ODC1 as representative hub proteins involved in redox regulation and metabolic adaptation. Furthermore, Western blotting confirmed increased GSTP2 expression following melittin treatment. Overall, these findings provide a comprehensive proteomic landscape of melittin-treated U14 cervical cancer cells and suggest that mitochondrial OXPHOS remodeling and redox-associated pathways may contribute to the antitumor activity of melittin.

cell biology↗

Neural mechanisms of musical syntax and tonality, and the effect of musicianship.

The neural basis for the processing of musical syntax has previously been examined almost exclusively in classical tonal music, which is characterized by strictly organized hierarchical structure. The present study investigated the neural mechanisms for processing musical syntax across genres varying in tonality - classical, impressionist, and atonal music - and, in addition, examined how musicianship modulates such processing. Results showed that, first, the dorsal stream, including bilateral inferior frontal gyrus and superior temporal gyrus, plays a key role in the perception of tonality. Second, right fronto-temporal regions were crucial in allowing musicians to outperform non-musicians in musical syntactic processing; musicians also benefit from a cortical-subcortical network including pallidum and cerebellum, suggesting more auditory-motor interaction in musicians than in non-musicians. Third, left pars triangularis carries out on-line computations independently of tonality and musicianship, whereas right pars triangularis is sensitive to tonality and partly dependent on musicianship. Finally, unlike tonal music, processing of atonal music could not be differentiated from that of scrambled notes, both behaviorally and neurally, even among musicians. The present study highlights the importance of studying varying music genres and experience levels, and provides a better understanding of musical syntax and tonality processing and how such processing is modulated by music experience.

neuroscience↗

Correlation between the epigenetic modification of histone H3K9 acetylation of NR2B gene promoter in rat hippocampus and ethanol withdrawal syndrome

Our results showed that, in rat hippocampus, the ethanol withdrawal syndrome score was increased at 2 h, peaked at 6 h after withdrawal of ethanol, and reduced to the level parallel to the normal control group at day 3 after ethanol withdrawal. The NR2B mRNA expression and protein levels of rat hippocampus region showed similar patterns. Further correlation analyses indicted that both histone H3K9 acetylation in NR2B gene promoter and the expression levels of NR2B were positively associated with ethanol withdrawal syndrome. Chronic ethanol exposure may result in epigenetic modification of histone H3K9 acetylation in NR2B gene promoter in rat hippocampus, and the expression levels of NR2B were found to be positively correlated with EWS.\n\nAbstractPrevious studies showed that an epigenetic modification of N-methyl-D-aspartate (NMDA) receptor, especially NMDA receptor 2B subunit (NR2B), was involved in the pathological process of ethanol withdrawal syndrome (EWS). However, the relationship between the epigenetic regulation of the NR2B gene in the rat hippocampus region and EWS were inconsistent. A rat model of chronic ethanol exposure was established. EWS score and the behavioral changes were recorded at different points in time. The NR2B expression levels and the histone H3K9 acetylation level in the NR2B gene promoter region were measured using qRT-PCR, Western blot, immunofluorescence and chromatin immunoprecipitation, respectively. Finally, the relationships between the epigenetic modification of histone H3K9 acetylation of NR2B gene promoter and EWS were examined. Our results showed that the EWS score was increased at 2 h, peaked at 6 h after withdrawal of ethanol, and reduced to the level parallel to the normal control group at day 3 after ethanol withdrawal. The NR2B mRNA expression and protein levels showed similar patterns. Further correlation analyses indicted that both histone H3K9 acetylation in NR2B gene promoter and the expression levels of NR2B were positively associated with EWS. Chronic ethanol exposure may result in epigenetic modification of histone H3K9 acetylation in NR2B gene promoter in rat hippocampus, and the expression levels of NR2B were found to be positively correlated with EWS.

neuroscience↗