bioRxiv2025
The rapid growth of high-dimensional biological data has necessitated advanced data fusion techniques to integrate and interpret complex multi-omics and longitudinal datasets. Shared and unshared structure across such datasets can be identified in an unsupervised manner with Advanced Coupled Matrix and Tensor Factorization (ACMTF), but this cannot be related to an outcome. Conversely, N-way Partial Least Squares (NPLS) is supervised and captures outcome-associated variation but cannot identify shared and unshared structure. To bridge the gap between data exploration and prediction, we introduce ACMTF-Regression (ACMTF-R), an extension of ACMTF that incorporates a regression step, allowing for the simultaneous decomposition of multi-way data while explicitly capturing variation associated with a dependent variable. We present a detailed mathematical formulation of ACMTF-R, including its optimisation algorithm and implementation. Through extensive simulations, we systematically evaluate its ability to recover a small y-related component shared between multiple blocks, its robustness to noise, and the impact of the tuning parameter ({pi}) which controls the balance between data exploration and outcome prediction. Our results demonstrate that ACMTF-R can robustly identify the y-related component, correctly identifying outcome-associated shared and distinct variation, distinguishing it from existing approaches such as NPLS and ACMTF. The development of ACMTF-R was motivated by a real-world dataset investigating how maternal pre-pregnancy BMI affects the human milk microbiome, human milk metabolome, and infant faecal microbiome. Emerging evidence suggests that inter-generational transfer of maternal obesity may affect multiple omics layers, highlighting the need to identify outcome-associated variation. The applicability of ACMTF-R is therefore validated by applying it to this multi-omics dataset. ACMTF-R successfully identifies novel mother-infant relationships associated with maternal pre-pregnancy BMI, underscoring its utility in multi-omics research. Our findings establish ACMTF-R as a versatile tool for multi-way data fusion, offering new insights into complex biological systems by integrating common, local, and distinct variation in the context of a dependent variable. Author SummaryIn recent years, biological research has been transformed by the rise of high-throughput technologies, allowing us to simultaneously measure multiple different data (genes, microbes, and metabolites) within the same subject. While these datasets hold great promise, analysing them in an integrated way remains challenging. Existing tools either focus on uncovering patterns in the data or on predicting outcomes, but rarely both. In this study, we present a new method called ACMTF-Regression (ACMTF-R), which combines these aspects. ACMTF-R helps researchers identify shared and distinct biological patterns across different datasets while also relating these patterns to specific outcomes. Using simulated data, we show that ACMTF-R can detect subtle signals that would otherwise go unnoticed. We also apply it to a real-world study of mothers and their infants, revealing how maternal obesity influences breast milk and gut microbes in the baby. Our approach provides a powerful new tool for studying complex biological systems and can be especially valuable in fields like microbiome research, metabolomics, and personalized medicine.