Biology subjects

tang, y.

Publications and source records attributed to tang, y..

4 recordsLinked to original sources

bioRxiv2023

ALOX15, a new potential functional target of lung adenocarcinoma

PurposeThe purpose of this study is to explore whether the down-regulation of ALOX15 is related to the stage, differentiation and prognosis of lung adenocarcinoma, whether the overexpression of ALOX15 can inhibit tumor proliferation and metastasis, and whether it is related to the functional target of lung adenocarcinoma(LUAD). MethodsFirstly, bioinformatics of lung cancer patients was analyzed using the TCGA database to study the differential expression of ALOX15 in LUAD and its effect on the survival of LUAD. Then, 50 clinical tissue samples of LUAD were collected to detect the expression of ALOX15 and its relationship with the differentiation degree and stage of lung cancer. Finally, the relationship between the expression of ALOX15 and the proliferation and differentiation of LUAD cell lines (NCI-H1944, A549, PC9) with different degrees of differentiation and the construction of ALOX15 overexpression stable lines was detected. ResultsALOX15 bioinformatics analysis showed that ALOX15 decreased significantly in the early stage of LUAD and had no correlation with the survival of lung cancer patients. ALOX15 was downregulated in LUAD with low differentiation and metastasis in LUAD. LUAD cell lines showed that the lower the degree of differentiation, the lower the expression of ALOX15, and the overexpression of ALOX15 in LUAD cells inhibited the proliferation and migration of cancer cells. ConclusionThese results suggest that the expression of ALOX15 is closely related to the differentiation, proliferation, and metastasis of LUAD, and that upregulation of ALOX15 may inhibit the development of LUAD, suggesting that ALOX15 is a potential biological therapeutic target. Author summaryO_ST_ABSWhy was this study done?C_ST_ABSO_LILung cancer is one of the most frequently diagnosed cancers in the world and the leading cause of cancer-related deaths worldwide. C_LIO_LILung cancer is a heterogeneous disease with a wide range of clinicopathologic features. Lung cancer is roughly divided into non-small cell lung cancer (85% of all diagnoses) and small cell lung cancer (15% of all diagnoses). Adenocarcinoma is a common subtype of non-small cell lung cancer, and its recurrence rate is high, and the prognosis is poor. Therefore, the pathogenesis and characteristics of adenocarcinoma are studied and explored. C_LI What did the researchers do and find?O_LIBiological information database was used to analyze the expression level of arachidonic acid-15-lipoxygenase (ALOX15) in lung adenocarcinoma, and then the expression differences were discussed through clinical samples and cell experiments. C_LIO_LILow ALOX15 expression was detected in lung adenocarcinoma (LAUD) patients compared with normal tissues, and ALOX15 levels influenced LUAD development. C_LIO_LIBy infecting A549 with lentivirus and overexpressing ALOX15 of A549 and PC-9 with PC9, it was found that ALOX15 inhibited the proliferation of tumor cells C_LI What do these findings mean?O_LIarachidonic acid-15-lipoxygenase may be one novel potential biomarker for LUAD and a potential therapeutic target C_LI

cancer biology↗

bioRxiv2019

Delineating the Organization of Projection Neuron Subsets with Multi-fluorescent Rabies Virus Tracing Tool

The elegant functions of the brain are facilitated by sophisticated connections between neurons, the architecture of which is frequently characterized by one nucleus connecting to multiple targets via projection neurons. Delineating the sub-nucleus fine architecture of projection neurons in a certain nucleus could greatly facilitate its circuit, computational, and functional resolution. Here, we developed multi-fluorescent rabies virus to delineate the fine organization of corticothalamic projection neuron subsets in the primary visual cortex (V1). By simultaneously labeling multiple distinct subsets of corticothalamic projection neurons in V1 from their target nuclei in thalamus (dLGN, LP, LD), we observed that V1-dLGN corticothalamic neurons were densely concentrated in layer VI, except for several sparsely scattered neurons in layer V, while V1-LP and V1-LD corticothalamic neurons were localized to both layers V and VI. Meanwhile, we observed a fraction of V1 corticothalamic neurons targeting multiple thalamic nuclei, which was further confirmed by fMOST whole-brain imaging. We further conceptually proposed an upgraded sub-nucleus tracing system with higher throughput (21 subsets) for more complex architectural tracing. The multi-fluorescent RV tracing tool can be extensively applied to resolve architecture of projection neuron subsets, with a strong potential to delineate the computational and functional organization of these nuclei.

neuroscience↗

bioRxiv2019

SK4 calcium-activated potassium channels activated by sympathetic nerves enhances atrial fibrillation vulnerability in a canine model of acute stroke

New-onset atrial fibrillation (AF) is common in patients with acute stroke (AS). Studies have shown that intermediate-conductance KCa channels (SK4) play an important role in cardiomyocyte automaticity. The aim of this study was to investigate the effects of SK4 on AF vulnerability in dogs with AS. Eighteen dogs were randomly divided into a control group, AS group and left stellate ganglion ablation (LSGA) group. In the control group, dogs received craniotomy without right middle cerebral artery occlusion (MCAO). AS dogs were established using a cerebral ischemic model with right MCAO. LSGA dogs underwent MCAO, and LSGA was performed. Three days later, the dispersion of the effective refractory period (dERP) and AF vulnerability in the AS group were significantly increased compared with those in the control group and LSGA group. However, no significant difference in dERP and AF vulnerability was found between the control group and the LSGA group. The SK4 inhibitor (TRAM-34) completely inhibited the inducibility of AF in AS dogs. SK4 expression and levels of noradrenaline (NE), {beta}1-AR, p38 and c-Fos in the atrium were higher in the AS dogs than in the control group or LSGA group. However, no significant difference in SK4 expression or levels of NE, {beta}1-AR, p38 and c-Fos in the left atrium was observed between the control group and LSGA group. SK4 plays a key role in AF vulnerability in a canine model with AS. The effects of LSGA on AF vulnerability were associated with the p38 signaling pathways.

neuroscience↗

bioRxiv2019

The co-stimulation of anti-CD28 and IL-2 enhances the sensitivity of ELISPOT assays for detection of neoantigen-specific T cells in PBMC

Neoantigen-based cancer immunotherapies hold the promise of being a truly personalized, effective treatment for diverse cancer types. ELISPOT assays, as a powerful experimental technique, can verify the existence of antigen specific T cells to support basic clinical research and monitor clinical trials. However, despite the high sensitivity of ELISPOT assays, detecting immune responses of neoantigen specific T cells in a patient or healthy donors PBMCs is still extremely difficult, since the frequency of these T cells can be very low. We developed a novel experimental method, by co-stimulation of T cells with anti-CD28 and IL-2 at the beginning of ELISPOT, to further increase the sensitivity of ELISPOT and mitigate the challenge introduced by low frequency T cells. Under the optimal concentration of 1g/mL for anti-CD28 and 1U/ml for IL-2, our new method can increase sensitivity by up to 5 folds comparing to a conventional ELISPOT, and outperforms other cytokine stimulation alternatives. To the best of our knowledge, this is the first report that the co-stimulation of anti-CD28 and IL-2 is able to significantly improve the sensitivity of ELISPOT assays, indicating that anti-CD28 and IL-2 signaling can act in synergy to lower the T cell activation threshold and trigger more neoantigen-specific T cells.

immunology↗